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Metagenomics

16S rRNA Gene Sequencing

Targeted bacterial microbiome profiling using 16S rRNA amplicon sequencing

Turnaround Time~2 weeks
🧪Specimen TypeOther
🔬Method16S rRNA V1-V2 amplicon sequencing
16S rRNA Gene Sequencing

This test analyzes the 16S ribosomal RNA gene, a highly conserved genetic marker found in bacteria. By sequencing specific regions of this gene, it enables accurate identification and classification of bacterial species present in a sample. The method is widely used in microbiome research, clinical diagnostics, and environmental studies to profile microbial communities, detect pathogens, and study bacterial diversity. Results provide insights into the composition and relative abundance of bacteria, supporting both clinical decision-making and scientific investigations.

  • Identification and classification of bacterial species in clinical, environmental, or research samples
  • Microbiome profiling to assess bacterial diversity and relative abundance
  • Detection of uncommon or difficult-to-culture bacteria
⚠ Not intended for:

-Identification of viruses, fungi, or parasites (non-bacterial organisms) -Determining antibiotic susceptibility or resistance directly -Providing quantitative bacterial load measurements -Diagnosing conditions where bacterial presence is not relevant

Specimen Type: Other
Preferred Collection:

For preferred collection, please contact our staff on a case‑by‑case basis.

Rejection Criteria:
  • Samples with insufficient quantity or poor quality of DNA
  • Specimens contaminated or improperly labeled
  • Degraded samples due to improper storage or transport
  • Non-bacterial samples submitted without clear indication of relevance
Shipping Instructions:

-For preferred collection, please contact our staff on a case‑by‑case basis. -Completed test requisition form. Address: Unit 220, 2/F, Building 16W, HKSTP, Pak Shek Kok, NT, Hong Kong.

Please contact us for pricing.

  • Results indicate bacterial taxa present in the sample, often at genus level depending on sequence resolution.
  • Relative abundance data can highlight dominant or rare organisms.
  • Clinical interpretation requires correlation with patient history, symptoms, and other laboratory findings, as presence of bacteria does not always imply pathogenicity.

The 16S ribosomal RNA gene is highly conserved across bacterial species, with variable regions that allow differentiation between taxa. Sequencing this gene provides a culture-independent method for bacterial identification. It is particularly useful when traditional culture methods fail or when complex microbial communities need to be studied, such as in gut microbiome research or recurrent infections.